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vector control  (Addgene inc)


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    Addgene inc vector control
    Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+control+vector/pBABE-hygro+(Plasmid+%231765)/pm40754831-267-20-23
    Average 94 stars, based on 69 article reviews
    vector control - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: PIK3CA hotspot mutations differentially impact responses to MET targeting in MET-driven and non-driven preclinical cancer models
    Article Snippet: .. NIH3T3 MET M1268T cells were transfected using standard reverse transfection protocols with Lipofectamine 2000 (Invitrogen), using the following plasmids: PIK3CA E545K and H1047R (Addgene plasmids #12524 and #12525 respectively, from Jean Zhao [ ]), as well as pBABE control vector (Addgene plasmid #14738,from Adrienne Cox [ ]). ..

    Article Title: Pancancer modelling predicts the context-specific impact of somatic mutations on transcriptional programs
    Article Snippet: .. Cal27 cell lines were transfected with pbabe control vector, pbabe WT PIK3CA and pbabe E545K PIK3CA vectors (Addgene) using Lipofectamine 3000 according to the manufacturer's instructions. .. Total RNA was extracted from MCF7 and Cal27 cell lines using an RNA Extraction Kit from Qiagen. cDNA synthesis was performed using iScript from Bio-Rad, according to the manufacturer's instructions.

    Control:

    Article Title: PIK3CA hotspot mutations differentially impact responses to MET targeting in MET-driven and non-driven preclinical cancer models
    Article Snippet: .. NIH3T3 MET M1268T cells were transfected using standard reverse transfection protocols with Lipofectamine 2000 (Invitrogen), using the following plasmids: PIK3CA E545K and H1047R (Addgene plasmids #12524 and #12525 respectively, from Jean Zhao [ ]), as well as pBABE control vector (Addgene plasmid #14738,from Adrienne Cox [ ]). ..

    Article Title: Pancancer modelling predicts the context-specific impact of somatic mutations on transcriptional programs
    Article Snippet: .. Cal27 cell lines were transfected with pbabe control vector, pbabe WT PIK3CA and pbabe E545K PIK3CA vectors (Addgene) using Lipofectamine 3000 according to the manufacturer's instructions. .. Total RNA was extracted from MCF7 and Cal27 cell lines using an RNA Extraction Kit from Qiagen. cDNA synthesis was performed using iScript from Bio-Rad, according to the manufacturer's instructions.

    Plasmid Preparation:

    Article Title: PIK3CA hotspot mutations differentially impact responses to MET targeting in MET-driven and non-driven preclinical cancer models
    Article Snippet: .. NIH3T3 MET M1268T cells were transfected using standard reverse transfection protocols with Lipofectamine 2000 (Invitrogen), using the following plasmids: PIK3CA E545K and H1047R (Addgene plasmids #12524 and #12525 respectively, from Jean Zhao [ ]), as well as pBABE control vector (Addgene plasmid #14738,from Adrienne Cox [ ]). ..

    Article Title: Pancancer modelling predicts the context-specific impact of somatic mutations on transcriptional programs
    Article Snippet: .. Cal27 cell lines were transfected with pbabe control vector, pbabe WT PIK3CA and pbabe E545K PIK3CA vectors (Addgene) using Lipofectamine 3000 according to the manufacturer's instructions. .. Total RNA was extracted from MCF7 and Cal27 cell lines using an RNA Extraction Kit from Qiagen. cDNA synthesis was performed using iScript from Bio-Rad, according to the manufacturer's instructions.



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    A–C. CD44 knockdown experiments. Du145 cells infected with the control (NS) or CD44 shRNA lentivectors (MOI 20; 72 h) were plated (10,000/well) in triplicate, pulsed by BrdU for 4 h, and processed for BrdU staining (see ). Shown in (A) is the quantification of BrdU + cells from a total of 500 cells counted for each and in (B) are representative images (×400). Inset, Western blot showing reduced CD44 protein expression in Du145 cells infected with CD44-shRNA (lane 2) compared with the cells infected with NS-shRNA (lane 1). For experiments in C, Du145 cells infected with the control (NS) or CD44 lentivector were plated (5,000/well) in quadruplicate on day 0. At the end of day 2, 4, 6, and 8, cells were dissociated and counted by Trypan blue exclusion. Plotted are the live cell numbers (mean ± SD) as a function of time. * P = 0.019; ** P = 0.003 (Student's t -test). D–F. CD44 overexpression experiments. Du145-VP16 cells infected with <t>GFP</t> or CD44 retroviral vectors (MOI 20; 72 h) were plated (10,000/well) in triplicate, pulsed by BrdU for 4 h, and processed for BrdU staining. Shown in (D) is the quantification of BrdU + cells from a total of 500 cells counted for each condition and in (E) are representative images (×400). Inset, Western blot showing increased CD44 protein expression in Du145-VP16 cells infected with <t>pBabe-CD44</t> (lane 2) compared with the cells infected with pBabe-GFP (lane 1). For experiments in F, Du145-VP16 cells infected with the GFP or CD44 retroviral vectors were plated (5,000/well) in quadruplicate on day 0. At the end of day 2, 4, 6, and 8, cells were dissociated and counted by Trypan blue exclusion. Plotted are the live cell numbers (mean ± SD) as a function of time. *P = 0.035; **P = 0.028. G–H. Paclitaxel inhibits PC3 orthotopic tumor growth (G) and metastasis (H). Presented in (G) is the tumor weight (mean ± S.D; n = 5 for each group). Tumor incidence is the same (i.e., 5/5) for both groups. Shown in (H) are representative phase and GFP microphotographs of lung metastases, i.e., GFP + foci.
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    Image Search Results


    A–C. CD44 knockdown experiments. Du145 cells infected with the control (NS) or CD44 shRNA lentivectors (MOI 20; 72 h) were plated (10,000/well) in triplicate, pulsed by BrdU for 4 h, and processed for BrdU staining (see ). Shown in (A) is the quantification of BrdU + cells from a total of 500 cells counted for each and in (B) are representative images (×400). Inset, Western blot showing reduced CD44 protein expression in Du145 cells infected with CD44-shRNA (lane 2) compared with the cells infected with NS-shRNA (lane 1). For experiments in C, Du145 cells infected with the control (NS) or CD44 lentivector were plated (5,000/well) in quadruplicate on day 0. At the end of day 2, 4, 6, and 8, cells were dissociated and counted by Trypan blue exclusion. Plotted are the live cell numbers (mean ± SD) as a function of time. * P = 0.019; ** P = 0.003 (Student's t -test). D–F. CD44 overexpression experiments. Du145-VP16 cells infected with GFP or CD44 retroviral vectors (MOI 20; 72 h) were plated (10,000/well) in triplicate, pulsed by BrdU for 4 h, and processed for BrdU staining. Shown in (D) is the quantification of BrdU + cells from a total of 500 cells counted for each condition and in (E) are representative images (×400). Inset, Western blot showing increased CD44 protein expression in Du145-VP16 cells infected with pBabe-CD44 (lane 2) compared with the cells infected with pBabe-GFP (lane 1). For experiments in F, Du145-VP16 cells infected with the GFP or CD44 retroviral vectors were plated (5,000/well) in quadruplicate on day 0. At the end of day 2, 4, 6, and 8, cells were dissociated and counted by Trypan blue exclusion. Plotted are the live cell numbers (mean ± SD) as a function of time. *P = 0.035; **P = 0.028. G–H. Paclitaxel inhibits PC3 orthotopic tumor growth (G) and metastasis (H). Presented in (G) is the tumor weight (mean ± S.D; n = 5 for each group). Tumor incidence is the same (i.e., 5/5) for both groups. Shown in (H) are representative phase and GFP microphotographs of lung metastases, i.e., GFP + foci.

    Journal: PLoS ONE

    Article Title: Drug-Tolerant Cancer Cells Show Reduced Tumor-Initiating Capacity: Depletion of CD44 + Cells and Evidence for Epigenetic Mechanisms

    doi: 10.1371/journal.pone.0024397

    Figure Lengend Snippet: A–C. CD44 knockdown experiments. Du145 cells infected with the control (NS) or CD44 shRNA lentivectors (MOI 20; 72 h) were plated (10,000/well) in triplicate, pulsed by BrdU for 4 h, and processed for BrdU staining (see ). Shown in (A) is the quantification of BrdU + cells from a total of 500 cells counted for each and in (B) are representative images (×400). Inset, Western blot showing reduced CD44 protein expression in Du145 cells infected with CD44-shRNA (lane 2) compared with the cells infected with NS-shRNA (lane 1). For experiments in C, Du145 cells infected with the control (NS) or CD44 lentivector were plated (5,000/well) in quadruplicate on day 0. At the end of day 2, 4, 6, and 8, cells were dissociated and counted by Trypan blue exclusion. Plotted are the live cell numbers (mean ± SD) as a function of time. * P = 0.019; ** P = 0.003 (Student's t -test). D–F. CD44 overexpression experiments. Du145-VP16 cells infected with GFP or CD44 retroviral vectors (MOI 20; 72 h) were plated (10,000/well) in triplicate, pulsed by BrdU for 4 h, and processed for BrdU staining. Shown in (D) is the quantification of BrdU + cells from a total of 500 cells counted for each condition and in (E) are representative images (×400). Inset, Western blot showing increased CD44 protein expression in Du145-VP16 cells infected with pBabe-CD44 (lane 2) compared with the cells infected with pBabe-GFP (lane 1). For experiments in F, Du145-VP16 cells infected with the GFP or CD44 retroviral vectors were plated (5,000/well) in quadruplicate on day 0. At the end of day 2, 4, 6, and 8, cells were dissociated and counted by Trypan blue exclusion. Plotted are the live cell numbers (mean ± SD) as a function of time. *P = 0.035; **P = 0.028. G–H. Paclitaxel inhibits PC3 orthotopic tumor growth (G) and metastasis (H). Presented in (G) is the tumor weight (mean ± S.D; n = 5 for each group). Tumor incidence is the same (i.e., 5/5) for both groups. Shown in (H) are representative phase and GFP microphotographs of lung metastases, i.e., GFP + foci.

    Article Snippet: Briefly, retroviral vectors, including control vector pBabe-GFP and pBabe-CD44 (Addgene, Cambridge, MA) were transfected into the Ampho-Phoenix 293 cells (ATCC).

    Techniques: Infection, shRNA, BrdU Staining, Western Blot, Expressing, Over Expression